基于电感耦合等离子体发射光谱法测定植物样品中多元素的前处理方法研究

doi:10.13686/j.cnki.dzyzy.2023.04.015

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摘要在农业生态环境地球化学调查与评价中，植物的化学组成是重要的直接指标，因此对植物样品元素含量进行准确测定具有重要意义.本研究的主要目的是开发一种利用电感耦合等离子体发射光谱（ICP-OES）测定植物样品中多元素的分析方法，同时不需要在光谱测定之前进行费力而繁琐的样品前处理.采用微波消解、电热板消解及超声消解3种方法处理植物样品，利用ICP-OES分别测定植物样品中Ca、Cr、Cu、Fe、Mg、Mn、Ni、Pb、Sr、Zn等10种元素的含量，并对3种方法的消解效果进行比较.结果表明：3种方法的检出限分别为0.042×10^-6~0.239×10^-6、0.046×10^-6~0.237×10^-6、0.026×10^-6~0.232×10^-6；测定待测元素的相对标准偏差均低于10%；相对误差分别为-11%~+13.8%，-22.1%~+8.7%，-13.3%~+8.9%.其中超声消解表现出更低的检出限和较好的准确度，满足DD2005-03《生态地球化学评价样品分析技术要求（试行）》.同时验证了超声消解在实际工作中的可行性.实验证明，王水溶样超声辅助消解是一种简单、快速、准确的样品制备方法，能够满足植物样品分析测试要求.

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关键词 ：植物样品, 电感耦合等离子体发射光谱法, 微波消解, 电热板消解, 超声消解, 样品前处理

Abstract：The chemical composition of plants is as an important direct indicator in the geochemical survey and evaluation of agroecological environment. It is of great significance to accurately determine the element content of plant samples. The main purpose of this study is to develop an analytical process for the determination of multiple elements in plant samples using inductively coupled plasma optical emission spectrometry (ICP-OES) without the need for tedious sample pretreatment prior to spectroscopic determination. The plant samples are treated by three methods including microwave digestion, electrothermal plate digestion and ultrasonic digestion. The contents of Ca, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Sr and Zn in plant samples are determined respectively by ICP-OES, with comparison of digestion effects of the three methods. The results reveal that the detection limits of three methods are 0.042×10^-6-0.239×10^-6, 0.046×10^-6 -0.237×10^-6 and 0.026×10^-6-0.232×10^-6, respectively. The relative standard deviations of the elements to be measured are all lower than 10%, with the relative errors of -11% to +13.8%, -22.1% to +8.7%, and -13.3% to +8.9%, respectively. Ultrasonic digestion shows a lower detection limit and better accuracy, which meets the requirements of DD2005-03 Technical Requirements for Sample Analysis of Eco-geochemical Evaluation (Trial), and its feasibility in practice is verified. The test proves that dissolving samples in aquaregia with the assisted ultrasonic digestion is a simple, quick and accurate sample preparation method, which can meet the requirements of plant sample analysis.

Key words： plant sample ICP-OES microwave digestion electrothermal plate digestion ultrasonic digestion sample pretreatment

收稿日期: 2022-04-12

PACS:

P593

基金资助:中国地质调查局项目"太行山区山西段生态修复支撑调查"（编号DD20208069）.

通讯作者: 卢兵(1977-),男,高级工程师,主要从事自然资源分析测试工作,通信地址黑龙江省哈尔滨市南岗区保健副路1号,E-mail//530004701@qq.com E-mail: 530004701@qq.com

作者简介: 赵文志(1987-),男,博士,工程师,主要从事自然资源分析测试工作,通信地址黑龙江省哈尔滨市南岗区保健副路1号,E-mail//zhaowenzhi817@163.com

引用本文:

赵文志, 张填昊, 张元, 杨园, 卢兵. 基于电感耦合等离子体发射光谱法测定植物样品中多元素的前处理方法研究[J]. 地质与资源, 2023, 32(4): 505-511,516.
ZHAO Wen-zhi, ZHANG Tian-hao, ZHANG Yuan, YANG Yuan, LU Bing. A PRETREATMENT PROCESS FOR DETERMINATION OF MULTIPLE ELEMENTS IN PLANT SAMPLES BASED ON ICP-OES. GEOLOGY AND RESOURCES, 2023, 32(4): 505-511,516.

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http://manu25.magtech.com.cn/Jweb_dzyzy/CN/10.13686/j.cnki.dzyzy.2023.04.015 或 http://manu25.magtech.com.cn/Jweb_dzyzy/CN/Y2023/V32/I4/505

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